Flow Cytometry

Program Overview

The CDI offers a modern Flow Cytometry Core Facility that includes high parameter fluorescent activated cell sorting (FACS) and flow cytometry instruments and services. The Flow Cytometry Core Facility lends its expertise in their capabilities to the CDI community and provides consultation on experimental design and fluorescent panel development, as well as in-depth training to allow unsupervised usage.

Flow Cytometry Core’s Mission

The CDI Flow Cytometry Core Facility is focused on providing top-of-the-line instrumentation that will maximize scientific impact for all of its users. We communicate with our scientific community and partners to ensure that we continually deliver improvements and cutting edge, reliable services. The resources of the core facility are available to all investigators at the CDI and its affiliated institutions.

The Flow Cytometry Shared Resource (FCSR) at CDI is part of the National Cancer Institute-designated Georgetown Lombardi Comprehensive Cancer Center, and additional flow cytometry resources are available and can be accessed here.

Instrumentation and Services

Technical Services offered

• Guidance in experimental methodologies and design
• Technical assistance for grant proposals and scientific manuscripts
• Assay development to fit users’ research-specific needs
• Instrumentation and analysis software training

Cytometry Equipment available

• BD LSRFortessa flow cytometer analyzer configured for four LASER (405nm, 488nm, 561nm,640 nm), 16 color analysis (BD LSRFortessa specifications) located in Building 111 lab A422.
• BD FACSymphony A3 flow cytometer analyzer configured for five LASER (355nm, 405nm, 488nm, 561nm, 637nm), 28 color analysis (BD FACSymphony A3 specifications) located in Building 111 lab C521
• BD FACSAria III cell sorter configured for five LASER (355nm, 405nm, 488nm, 561nm, 640nm), 15 color analysis (BD FACS Aria III specifications). Sorting capabilities include four-way collection tubes, variable plate sizes, and single cell 96 well plates. ARIA III sorter is housed in a high containment BioBUBBLE benchtop enclosure located in Building 111 lab B421
• BD FACSMelody cell sorter configured for three LASER (488nm, 561nm, 640nm), 8 color analysis (BD FACSMelody specifications). Sorting capabilities include two-way collection tubes, variable plate sizes, and single cell 96 well plates. Melody sorter is housed in a BSL2 biosafety cabinet located in Buildings 111 lab A422.
• BD LSRFortessa flow cytometer analyzer configured for five LASER (355nm, 405nm, 488nm, 561nm, 640 nm), 18 color analysis (BD LSRFortessa specifications), HTS, located in Building 123 lab 5532.
• BD FACSMelody cell sorter configured for three LASER (405nm, 488nm, 640nm), 9 color analysis (BD FACSMelody specifications). Sorting capabilities include four-way collection tubes, variable plate sizes, and single cell 96 well plates. Melody sorter is housed in a BioBUBBLE benchtop enclosure located in Building 123 lab 5532.
• BD FACSymphony A5 SE flow cytometer analyzer configured for five LASER (355nm, 405nm, 488nm, 561nm, 637nm ), up to 48 detectors located in Building 111 lab C521.
• Spectradyne nCS1 microfluidic nanoparticle analyzer employs resistive pulse sensing method to size and count nanoparticles in a sample. nCS1 located in Building 111 lab C424.

Instrument Configurations

• BD LSRFortessa Cell Analyzer (5532) (PDF)
• BD LSRFortessa (A422) (PDF)
• BD FACSMelody Sorter (5532) (PDF)
• BD FACSMelody Sorter (A422) (PDF)
• BD FACSymphony A5 SE analyzer (PDF)
• BD FACSymphony A3 analyzer (PDF)
• BD FACSARIA III Sorter (PDF)

Analysis workstation:

A stand-alone workstation is available. The workstation is equipped with Flowjo, a flow cytometry data analysis software.

Key Faculty/Staff

Yuanyuan Tian
Core Manager, Flow Cytometry Suite
Postdoctoral Fellow, CDI
yuanyuan.tian@hmh-cdi.org
Yuanyuan Tian oversees the overall operations of the FCSR. Yuanyuan received her graduate training in Immunology from Shanghai Jiao Tong University and has 7 years of experience in cell analysis and cell sorting. Yuanyuan  is available for assay development and general experimental flow cytometry consultations.

Wen-Shan Tsao
Flow Cyto/Microscopy Lab Coordinator, CDI
wenshan.tsao@hmh-cdi.org
Wen-Shan Tsao, manages day-to-day operations of the FCSR. Wenshan  received her graduate training in Biotechnology from the National Dong Hwa University and has 8 years of experience in cell analysis and cell sorting. Tsao is available for instrument training, experiment troubleshooting, cytometric analysis, and cell sorting.